Cryptosporidium Oocyst Detection in Water Samples: Floatation Technique Enhanced with Immunofluorescence Is as Effective as Immunomagnetic Separation Method
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چکیده
منابع مشابه
Evaluation of immunomagnetic separation and the sucrose flotation methods coupled with immunofluorescence or PCR for detection of Cryptosporidium and Giardia (oo)cysts in water samples
Introduction: Detection of Cryptosporidium and Giardia parasites in water samples is usually performed by US Environmental Protection Agency 1623 method. Nevertheless, the USEPA1623 method still need improvement, to prevent and control the water borne parasitic disease. Therefore, we undertook the present study. Materials and methods: Totally 48 surface water samples were collected. Four sampl...
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Cryptosporidium parvum and C. hominis have been the cause of large and serious outbreaks of waterborne cryptosporidiosis. A specific and sensitive recovery-detection method is required for control of this pathogen in drinking water. In the present study, nested PCR-restriction fragment length polymorphism (RFLP), which targets the divergent Cpgp40/15 gene, was developed. This nested PCR detecte...
متن کاملevaluation of immunomagnetic separation and the sucrose flotation methods coupled with immunofluorescence or pcr for detection of cryptosporidium and giardia (oo)cysts in water samples
introduction: detection of cryptosporidium and giardia parasites in water samples is usually performed by us environmental protection agency 1623 method. nevertheless, the usepa1623 method still need improvement, to prevent and control the water borne parasitic disease. therefore, we undertook the present study. materials and methods: totally 48 surface water samples were collected. four sample...
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Immunofluorescence assay (IFA) and immunomagnetic electrochemiluminescence (IM-ECL) were used for comparison of the percent recovery of Cryptosporidium parvum in environmental water samples obtained from a spring draining a karst basin. The monoclonal antibodies to C. parvum, isotype IgG3 were used for optimization of the IM-ECL protocol. The combination of biotinylated and TAG-labeled anti-C. ...
متن کاملPerformances of the immunomagnetic separation method for Cryptosporidium in water under various operation conditions.
Immunomagnetic separation (IMS) has been specified as a standard method for the measurement of Cryptosporidium in some countries. In this study, the IMS method was evaluated on the basis of the recovery efficiencies of Cryptosporidium oocysts at various IMS operation conditions. The average recovery for different Cryptosporidium concentrations in deionized water was 82.6 +/- 18.2% (n = 52). No ...
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ژورنال
عنوان ژورنال: The Korean Journal of Parasitology
سال: 2009
ISSN: 0023-4001
DOI: 10.3347/kjp.2009.47.4.353